Taq error problems
Tue Jan 17 06:46:43 EST 1995
I'm using a PCR technique to detect mutations in genomic DNA
in order to quantitate the mutations I need to detect a very
small number of mutations above a Taq error background. I
therefore need to reduce the Taq error rate as much as possible. I
know about the addition of a proofreading polymerase, and am trying
that at present. Can anyone help with any other suggestions, e.g.
cycle number (I'm using 24), DNA concentration ( I'm using 10 microg's),
primer concentration (20 pM)?
Any help would be greatly appreciated
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