Think you're good at PCR? Try this one!
Mike Morris
mike at medsun.unige.ch
Tue Jan 17 11:27:13 EST 1995
I reckon it's magic. I certainly don't think the 450 bp fragment is
ss-DNA - that should run slower, shouldn't it? (it certainls does
in acryl. gels).
Could you be getting heterodimers, somehow? Are you amplifying a lot
of loci, or just a single rearrangement?
Mike
*******************************************************************
Division of Medical Genetics tel (Switzerland) (22) 702.56.94
CMU, University of Geneva fax (Switzerland) (22) 702.57.06
Geneva, Switzerland email mike at medsun.unige.ch
More information about the Methods
mailing list