metal chelate chromatography in urea

[dheeley at kean.ucs.mun.ca]

Our lab is interested in knowing whether it is feasible to perform metal 
chelate affinity chromatography in the presence of high concentrations of 
urea. The concern is that the urea will co-ordinate the immobilised metal 
ions & remove them from the resin. Has anyone tried to do this ?

Thanks,

D.Heeley
Dept Biochem
Memorial University
Newfoundland
Canada.