FITC-antibody labeling

lw75 Lynne_A_WHITEHEAD at UMAIL.UMD.EDU
Wed Jan 18 11:57:11 EST 1995


Hi there!

I was wondering if anyone knows the purpose behind the addition of the
NH4Cl and xylene cylanol/glycerol to FITC-labeled antibodies during
antibody labeling just prior to separating protein-bound and  -unbound
FITC  from the sample (proceedure from antibodies, published by cold springs
habor).
Any insight would be helpful!
Thanks!

Lynne Whitehead
U. of Maryland
Microbiology Dept.



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