thanks: diff display

Bruce J. Turner fishgen at vt.edu
Wed Jan 18 11:51:23 EST 1995



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Thanks to everyone who responded to my query re references for the diff.
display technique. A collection of replies is attached (I have tried to
eliminate overlaps).



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The originators of DD have started a company"GenHunter Corp, ph:617-739-6771.

I've been interested in doing DD, got their kit, but haven't tried it yet.

(Don't work for them)

let me know what else you find

Good Luck and Good Displays

rjc in denver










Liang and Pardee Science Vol 257:967 1992 Liang et al Nucleic Acids
research Vol 21(14):3269 1993 Bauer et al Nucleic Acids research Vol
21(18):4272 1993 **** The Best one**** Callard et al Biotechniques
Vol16(6):1096 1994 Also several others in Biotechniques in the last 6
months.

Cheers

--
Shahram Mori	_/\_
Program in Molecular Biology	_\ /_
Dept. of chemistry and Biochemistry Box 3C	\_ _/
NMSU Las Cruces NM	||
88003




>The "must" is of course, Liang, P. and Pardee A. B. 1992. Differential
display of eukaryotic messenger RNA by means of the polymerase chain
reaction. Science 257: 967-971.

Then: Liang, P., Averboukh, L. and Pardee, A. 1993. Distribution and
cloning of eukaryotic mRNAs by means of differential display: refinements
and optimization. Nucleic Acids Res. 21: 3269-3275.

Bauer, D., Muller, H., Reich, J., Riedel, H., Ahrenkiel, V., Warthoe, P.
and Strauss, M. 1993. Identification of differentially expressed mRNA
species by an improved display technique (DDRT-PCR) Nucleic Acids Res.
21:4272-4280.

There are two other important papers that must have been already published.
One is Liang et. al. in Methods Enzymol and the other by Bauer et al (it is
a very good protocol) in PCR methods and applications.

As an alternative to the Liang protocol, Welsh and collegues published in
1992, Arbitrarily primed PCR fingerprinting of RNA, Nucleic Acids Res, 20:
4965-4970.

I found out, that if you use the Welsh protocol with 17-mers instead of
20-mers, it gives a good pattern of bands. The advantage of this protocol
is that the fragments are longer, typically 500bp and they are not from the
3' end, which is a noncoding region.

I hope it helps!
 References Date: 18 Jan 1995 13:58:22 GMT
NNTP-Posting-Host: via-annex3-16.cl.msu.edu


1. Lebeau, M. C., G. Alvarezbolado, W. Wahli and S. Catsicas. PCR Driven
DNA-DNA Competitive Hybridization: A New Method for Sensitive Differential
Cloning. Nucleic Acids Res. 19:4778. 1991.

2. Liang, P. and A. B. Pardee. Differential display of eucaryotic messenger
RNA by means of the polymerase chain reaction. Science 257:967-971. 1992.

3. Scherba, G., L. Jin, W. M. Schnitzlein and M. H. Vodkin. Differential
Polymerase Chain Reaction for Detection of Wild- Type and a Vaccine Strain
of Aujeszky's Disease (Pseudorabies) Virus. J. Virol. Methods 38:131-143.
1992.

4. Bauer, D., H. Muller, J. Reich, H. Riedel, V. Ahrenkiel, P. Warthoe and
M. Strauss. Identification of differentially expressed mRNA species by an
improved display technique (DDRT-PCR). Nucleic Acids Res. 21:4272-4280.
1993.

5. Liang, P. and A. B. Pardee. Distribution and cloning of eucaryotic mRNAs
by means of differential display: refinements and optimization. Nucleic
Acids Res. 21:3269-3275. 1993.

6. Tenorio, A., J. E. Echevarria, I. Casas, J. M. Echevarria and E.
Tabares. Detection and typing of human herpesviruses by multiplex
polymerase chain reaction. J. Virol. Methods 44:261-269. 1993.

7. Callard, D., B. Lescure and L. Mazzolini. A method for the elimination
of false positives generated by the mRNA differential display technique.
BioTechniques. 16:1096-1103. 1994.

8. Chen, Z., K. Swisshelm and R. Sager. A cautionary note on reaction tubes
for differential display and cDNA amplification in thermal cycling.
Biotech. 16:1002-1006. 1994.

9. Sokolov, B. P. and D. J. Prockop. A rapid and simple PCR-based method
for isolation of cDNAs from differentially expressed genes. Nucleic Acids
Res. 22:4009-4015. 1994.

10. Reeves, S. A., M-P. Rubio and D. N. Louis. General method for PCR
amplification and direct sequencing of mRNA differential display products.
Biotech. 18:18-20. 1995.

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