Total or polyA

Tracy Aquilla aquilla at salus.med.uvm.edu
Thu Jan 19 11:11:52 EST 1995


In Article <MAILQUEUE-101.950118163242.320 at Oberon.HSC.Colorado.edu>,
COHRS-R at Oberon.HSC.Colorado.edu ("Randall J. Cohrs") wrote:
>Greetings
>
>Given 50 ug of total RNA, is it better to isolate poly[A] prior to 
>cDNA synthesis or not ?

I wouldn't try it. I have tried to purify polyA mRNA from 50ug of total RNA
before, and I never got any polyA back from the purification. The starting
amount is just too small. My 2mmol worth.
    Tracy



More information about the Methods mailing list