Total or polyA

Tracy Aquilla aquilla at
Thu Jan 19 11:11:52 EST 1995

In Article <MAILQUEUE-101.950118163242.320 at>,
COHRS-R at ("Randall J. Cohrs") wrote:
>Given 50 ug of total RNA, is it better to isolate poly[A] prior to 
>cDNA synthesis or not ?

I wouldn't try it. I have tried to purify polyA mRNA from 50ug of total RNA
before, and I never got any polyA back from the purification. The starting
amount is just too small. My 2mmol worth.

More information about the Methods mailing list