Green Fluo. Prot.

[William J. Buikema]

I've got GFP working well in Anabaena cells, a filamentous cyanobacteria.  At
low levels of expression it works well, and can be easily visualized in
a microscope.  Moderate to high level expression is rather toxic, although
it takes the cells a while to die, so that it is possible to see very
brightly fluorescing cells.  I think the toxicity may have something to
do with interference with photosynthesis, but it's just a guess at this 
point.

Bill

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William J. Buikema        Phone: (312)702-1081   e-mail: hetman at uchicago.edu
Dept. Mol. Genet. & Cell Biol.                   /  / --- --- /|/|  /|  /| /
Univ. of Chicago, 920 E. 58th St.               /--/ /-   /  / / | /-| / |/
Chicago, IL 60637 USA       Fax: (312)702-3172 /  / /__  /  /    |/  |/  /