5' RACE

Kerry Hull khull at physio.med.ualberta.ca
Thu Jan 19 17:16:02 EST 1995


Hello,

I am attempting to amplify the 5' portion of a cDNA using Frohman's
modified RACE protocol (Methods in Enzymology, 218: 341-356).  He
recommends using Centricon-100 spin filters to purify reverse-transcribed
cDNA from primers, etc.  Does anyone know if Quiagen PCR Purification
Kits or Promega PCR Purification kits will work instead? Or, who sells
Centricon-100 spin filters.  Any other helpful hints would be greatly
appreciated!

Thanks,
Kerry



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