Extra bands on plasmid prep.

kp pootanak at hermes.bc.edu
Thu Jan 19 10:27:00 EST 1995


In article <pootanak-180195165111 at bi16.bc.edu>, pootanak at hermes.bc.edu
(Kusol Pootanakit) wrote:
> 
> After I did the plasmid prep (by alkaline lysis--phenol/chloroform--ethanol
> extraction) and ran the gel, what I got was the plasmid band and and extra
> band that is around 9kb.  So I am wondering what could that 9kb band be.
> 
> thanks

The larger band cannot be the concatemer as it is still present when I
tried to linearize it.

thanks



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