cloning full-length cDNAs without libraries

[Michael Cooley]

Jon Nakamoto (jnakamot at pediatrics.medsch.ucla.edu) wrote:
: Thanks for the RACE reference, but you'll note that the procedure I
: described is a significant modification of the original procedure
: (reduced background due to use of an amine-modified adaptor which is
: used both for 5' and 3' RACE, and combination of the 5' and 3' RACE
: reactions to get the entire cDNA, using LD PCR).

: I've spent months trying to get 5' ends of some tough cDNAs, so if
: this procedure works I'll use it. But I refuse to overpay for a kit.
: I'll figure out the nitty gritty myself, but a literature reference
: would sure help.

: Jon Nakamoto
: Clinical Instructor of Pediatrics/Endocrinology, UCLA
: jnakamot at pediatrics.medsch.ucla.edu


Sounds like the only trick to this is the adapter. Why not have a similar 
adapter synthesized?


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