Non-imidazole His-Tag elution from Ni-columns?

John E. Blume blume_j at hanv01.dnet.sandoz.com
Thu Jan 19 06:33:08 EST 1995


Does anyone know of a non-column destroying method for eluting
His-Tagged proteins from Ni ion columns?

Imidazole is not very nice for our protein activity for some
reason or another.

TIA,

John E. Blume, PhD          Voice: 201.503.8439
Sandoz Research Institute     Fax: 201.503.6097
East Hanover, NJ            Email: blume at sandoz.com




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