PCR: Taq or Vent???

Duncan Clark Duncan at genesys.demon.co.uk
Thu Jan 19 03:54:34 EST 1995


In article: <3ffc5s$fak at dns1.NMSU.Edu>  smori at nmsu.edu (Shahram Mori) writes:

> If all the conditions are similar, the only thing I see ( apart from a bad
> batch of Taq ) is that Taq is degrading your primers. On this net I have heard
> that taq has primer degrading activity. I don't know the author's of this
> work. I sure would like to see the paper.

I too would like to see a paper on this 'cos knowing this enzyme v.well I
find it difficult to believe. I know it has a 5'3' exonuclease activity but
primer degradation is usually a problem with Vent and Pfu 'cos of their 3'-5'
exonuclease activity not 5-3.

Duncan


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