SSCP

Edwin Fink fink at clinbio.med.uni-muenchen.de
Fri Jan 20 05:44:26 EST 1995


>Reply-to:      ONNO.Myriam at univ-rennes1.fr[ONNO.Myriam]
>
>Is it possible to realize the SSCP technique with non radioactive labeled nucleotides. for example Dig-UTP ? Is it possible to detect the non radioactive products on a dried polyacrylamide gel ? 
>
>
>
>		THANKS 


Many others and we are using silver staining of the PAGE for doing 
SSCP. No labeled markers are necessary. Probably any silver staining
 method is applicable. We use D. Riesner et al., (1989) Electrophoresis 10:377-389. 

Edwin Fink
Edwin Fink
Department of Clinical Biochemistry
University of Munich
E-Mail: fink at clinbio.med.uni-muenchen.de
Tel.: +49 89  5160 2601
Fax:  +49 89  5160 4735



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