Smooth muscle cell-specific housekeeping genes?
aquilla at salus.med.uvm.edu
Fri Jan 20 14:21:10 EST 1995
In Article <Hsieh.1140924755A at oitnews.harvard.edu>, Hsieh at cvlab.harvard.edu
(Chung-Ming Hsieh) wrote:
>I am doing some RNA analyses to study several possible smooth muscle
>cell-specific gene expression among different tissues, such as in various
>portions of the digestive system and in the cardiovascular system. Despite
>I loaded the same amount of total RNA in Northern analysis, it is hard to
>determine how much of the RNA really came out from the smooth muscle cells
>in each particular tissue/organ since most tissues are comprised of many
>different cell types.
>I am wondering if one can find a smooth muscle cell-specific housekeeping
>gene and use it as a probe to control the smooth muscle cell RNA proportion
>in various tissues. I have looked into alpha-smooth muscle actin and it is
>not expressed at the same level in the digestive system and the vascular
>The other solution to this is to do in situ hybridization, but that's
>Thanks in advance for your input!
>Hsieh at cvlab.harvard.edu
>Cardiovascular Biology Laboratory
>Harvard School of Public Health
Probably the best standard for your situation would be either 18S or 28S rRNA.
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