Crosslinking GST fusion proteins to glutathione beads

AShaw5 ashaw5 at
Fri Jan 20 22:34:17 EST 1995

We have had very poor luck crosslinking GSt fusion proteins to glutathione
beads.  The crosslinking appears to go OK but the beads are incredibly
sticky and useless in our binding assays.  So we routinely elute, desalt
and then couple to AFFigel.
Andrey Shaw

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