Competent BL21 Cells?

AShaw5 ashaw5 at aol.com
Fri Jan 20 22:18:29 EST 1995


When I am using BL21 cells, I am generally transferring a plasmid from DH5
so that I can get PET expression.  In these cases, the efficiency of the
bugs does not have to be very good.  So I make competent bugs using the
PEG/DMSO method first reported in PNAS which is also described in the Red
book.  It is a simple one step procedure and generally works.
Andrey Shaw



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