Competent BL21 Cells?

AShaw5 ashaw5 at
Fri Jan 20 22:18:29 EST 1995

When I am using BL21 cells, I am generally transferring a plasmid from DH5
so that I can get PET expression.  In these cases, the efficiency of the
bugs does not have to be very good.  So I make competent bugs using the
PEG/DMSO method first reported in PNAS which is also described in the Red
book.  It is a simple one step procedure and generally works.
Andrey Shaw

More information about the Methods mailing list