PCR product sequencing

bckraev at aeolus.ethz.ch bckraev at aeolus.ethz.ch
Fri Jan 20 10:39:33 EST 1995

The best kit in my hands was Sequitherm Long Read, which I adapted for use
with 33p-labelled primers ( sold by Epicentre Technologies ), however,
Sequenase is also OK, but it needs enormous amounts of DNA compared to
any cycle-sequencing kit. In both cases the PCR products were either gel
purified or passed over Qiagen PCR Kit columns. 

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