Needed:Low MW GFC Std.

Mark Jason Logan mjlogan at whale.st.usm.edu
Sun Jan 22 21:37:07 EST 1995


I am looking for a low MW protein to use as a std for gel filtration
chromatograpy.  The lowest one I have found is aprotinin (6,500), I really
need one around 3,200 Da.  I have tried the B-chain of insulin but it
elutes before aprotinin. I am currently using a 0.05M KPO4 (pH 3.72)
buffer.  In the next couple of days I will probably increase the salt
content to  see if that makes insulin behave any better.  In the mean time
I welcome any suggestions that you may have.


Please respond by e-mail to mjlogan at whale.st.usm.edu

Thanks in advance

Mark



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