Actin and GADPH Probes required
aquilla at salus.med.uvm.edu
Mon Jan 23 15:48:48 EST 1995
In Article <saleem.6.00364B3E at rfhsm.ac.uk>, saleem at rfhsm.ac.uk (Dr Saleem
>I would be most grateful if anybody could supply me with b-Actin and GADPH DNA
>If anybody could suggest alternatives to these traditional housekeeping genes
>for use as controls in Northern blots, this would be most appreciated.
>Dr Saleem Mohammed,
>Dept of Biochemistry & Molecular Biology,
>Royal Free Hospital School of Medicine.
>Rowland Hill Street,
>London. NW2 2PF.
I don't have any clones available, but these DNA templates may be purchased
pre-linearized (Ambion). Depending on your experiments, one of the rRNAs amy
be a better internal standard. I have found both b-actin and GAPDH to be
induced over 50% in various experiments.
More information about the Methods