Semi-Dry blotting problem
aquilla at salus.med.uvm.edu
Mon Jan 23 15:48:10 EST 1995
In Article <asch-2001951456530001 at mac102219.med.cornell.edu>,
asch at mail.med.cornell.edu wrote:
>In article <D2oFF4.A2z at ncifcrf.gov>, pnh at fcsparc6.ncifcrf.gov (Paul N
>>I find it's easy to place a membrane on top of a gel, but
>> the gel on top of the membrane gives me fits! Is there a slick hands-on trick
>> for doing this?
>I generally place the membrane on top of the gel and then place the wet
>filter paper layer on top of that and flip the whole mess over carefully
>onto my hand. The glass can then be separated from the gel by carefully
>lifting the plate and allowing the gel, membrane and bottom layer of
>filter paper to hang down a bit at one corner. Once the bottom layers are
>placed on the blotting apparatus, you can place the top layer of wet
>filter paper and go ahead and blot. I use a different brand semi-dry
>apparatus so I am not sure that the electrodes are oriented in the same
>fashion. If necessary, you could easily flip the 'sandwhich'.
>asasch at mail.med.cornell.edu (Adam S. Asch)
Try a kitchen spatula.
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