Semi-Dry blotting problem

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Jan 23 15:48:10 EST 1995


In Article <asch-2001951456530001 at mac102219.med.cornell.edu>,
asch at mail.med.cornell.edu wrote:
>In article <D2oFF4.A2z at ncifcrf.gov>, pnh at fcsparc6.ncifcrf.gov (Paul N
>Hengen) wrote:
>
>>I find it's easy to place a membrane on top of a gel, but
>> the gel on top of the membrane gives me fits! Is there a slick hands-on trick
>> for doing this?
>
>I generally place the membrane on top of the gel and then place the wet
>filter paper layer on top of that and flip the whole mess over carefully
>onto my hand.  The glass can then be separated from the gel by carefully
>lifting the plate and allowing the gel, membrane and bottom layer of
>filter paper to hang down a bit at one corner.  Once the bottom layers are
>placed on the blotting apparatus, you can place the top layer of wet
>filter paper and go ahead and blot.  I use a different brand semi-dry
>apparatus so I am not sure that the electrodes are oriented in the same
>fashion.  If necessary, you could easily flip the 'sandwhich'.
>
>-- 
>asasch at mail.med.cornell.edu (Adam S. Asch)

Try a kitchen spatula.
    Tracy



More information about the Methods mailing list