Best E.coli strain(s) for GST-expression (re. minimum proteolysis)

Jim Litts jlitts at onyx-pharm.com
Mon Jan 23 17:54:43 EST 1995


I have one particular protein that, when expressed as a GST-fusion protein,
has a great deal more difficulty with degradation than others.  I was
wondering what cell lines people are working with that might be useful for
stabilizing such proteins, hopefully while still maintaining a reasonable
level of expression.  Perhaps with endogenous protease deletions.  It seems
I remember having some such lines several years back in another lab, but I
can't find my records of them.  If you respond by Email, I will summarize
and post the information.  Thanks.

Jim



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