P1 sequencing protocol??

John W. Longshore Gene009 at uabdpo.dpo.uab.edu
Mon Jan 23 14:33:22 EST 1995


   I'm having problems sequencing a P1 clone that I have.  I'm using cycle
sequencing as suggested by the protocol I am following.  Can anyone help
me determine the correct primer to template ratio for the reactions?

Also, any P1 sequencing or subcloning protocols would be appreciated..

TIA, 

John

-- 
John W. Longshore
Laboratory of Medical Genetics
University of Alabama at Birmingham
gene009 at uabdpo.dpo.uab.edu



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