P1 sequencing protocol??

John W. Longshore Gene009 at uabdpo.dpo.uab.edu
Mon Jan 23 14:33:22 EST 1995

   I'm having problems sequencing a P1 clone that I have.  I'm using cycle
sequencing as suggested by the protocol I am following.  Can anyone help
me determine the correct primer to template ratio for the reactions?

Also, any P1 sequencing or subcloning protocols would be appreciated..



John W. Longshore
Laboratory of Medical Genetics
University of Alabama at Birmingham
gene009 at uabdpo.dpo.uab.edu

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