Homegrown Streptavidin

ANDREI POPOV ANDREI.POPOV at afrc.ac.uk
Tue Jan 24 19:05:26 EST 1995




Hi Dennis,

Ive done this once long ago, and I remember
one pitfall: the medium for St.avidinii should NOT
contain biotin for obvious reasons...
If you get the right strain purification is easy 
on a column with an analogue of biotin (if I dont mind
it's name is iminobiotin) with has lower affinity
for avidin

Best wishes

Andrei




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