Transfections w/ Promega's Transfectam
pmclean at darwin.bu.edu
Tue Jan 24 17:00:38 EST 1995
I have been trying to find a technique for transfections into primary rat
cerebellar neurons. So far I have tried CaPO4 precipitation, DEAE dextran
and lipofectin without success. Recently I realized that promega's
"Transfectam" reagent claims to give efficient transfection into the
aforementioned cell type. However in my hands it doesn't. I have varied
all parameters suggested and had endless discussions with their technical
So, I need help!!! Has anyone out there used transfectam successfully, in
particular w/ rat cerebellar neurons ? Or, does anyone have any other
methods of transfecting into these stubborn cells ? The only method in
the literature uses a lipopolyamine and this has recently been marketed as
Thanks for your help!
"Welcome to all things Scottish. If it's not Scottish, it's CRAP!"
-Mike Myers, Saturday Nite Live.
e-mail: pmclean at darwin.bu.edu
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