MAXIMUM PCR DNA TEMPLATES?

BCRUBIN at weizmann.weizmann.ac.il BCRUBIN at weizmann.weizmann.ac.il
Tue Jan 24 12:40:53 EST 1995


I had the same problams. Heard 2 seperate explanations: 1) too much DNA causes
unspecific anealing -> too little specific anealing -> no products. 2) Your DNA
 sample is not pure of salts,phenol and other Taq unfreindly stuff, and the mor
e you put of it, the more you inhibit Taq.
   Eitan.



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