leach at bu.edu
Tue Jan 24 11:16:30 EST 1995
In article <Reon.2.2F24AF0B at INFRUIT1.AGRIC.ZA>, Reon at INFRUIT1.AGRIC.ZA
(Reon Brand ) wrote:
> We need to perform a luciferase assay on extracts of transgenic plants
> expressing the Firefly luciferase gene. We do not have access to a
> luminometer. Any suggestions on which is the best kit to use (we've heard
> that Promega is marketing a new kit that includes co-enzymeA which improves
> the kinetics of the reaction)? Also, which instruments apart from
> luminometers can be used for the assay (we've believe that scintillation
> counters can also be used)?
> Thanx for any forthcoming tips and suggestions.
> Reon Brand & Heinrich Muller
one person that would be good for you to talk to regarding transfenic
plants is Steve Kay...of Virginia (email: sak5y at virginia.edu) who works on
circadian rhythms in arabidopsis.
Regarding a detector...we use a scintillation counter that has an added
single photon monitor..the latter enabling us to measure lucif
activity...we obtained our machine from Beckmann.
..... Martin Leach Email:leach at bu.edu
_|____ Dept. of Pharmacology Phone: (617) 638-5323
/ o / Boston Univ. School of Med. Fax: (617) 638-4329
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p.s. try BioMOO (virtual biology on the internet - telnet
My home page: http://184.108.40.206/Leach.html
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