direct seq of pcr

Dean R. Appling appling at utbc01.cm.utexas.edu
Tue Jan 24 10:12:07 EST 1995


Has anyone had any experience with the recently published method:
(Khorana, S., Gagel, R. F., and Cote, G. J. (1994) Direct sequencing of
PCR products in agarose gel slices. Nucl. Acids Res. 22, 3425-3426)?  We
have not been able to get clean sequence using this method.  Any helpful
tips will be appreciated.
_____________________________________
Dean R. Appling
Dept. of Chemistry & Biochemistry
Univ. of Texas at Austin
Austin, TX 78712-1167
(512) 471-5842 (voice)
(512) 471-5849 (fax)
appling at utbc01.cm.utexas.edu
_____________________________________



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