Distinguishing stringent from relaxed?

M.J. Pallen mbpln at s-crim1.dl.ac.uk
Wed Jan 25 13:57:40 EST 1995

I  am in the process of transduing a Tn10 relA mutation from one strain of 
Salmonella into another. Does anyone have a handy way of scoring the 
relA-negative phenotype, so that I can check that I really do have a relA 
mutation in the second strain? Cahel & Rudd in Neidhardt's E. coli & S. 
typhimurium book mention SMG medium. Is this the best way of 
distinguishing stringent from relaxed strains?

Dr Mark Pallen, Senior Lecturer in Medical Microbiology, 
St Bartholomew's Hospital Medical College, London, EC1A 7BE
currently at the Dept of Biochemistry, Imperial College, London, SW7 2AY 
email:m.pallen at ic.ac.uk  
phone: day ++44(0)1715945254, eves ++44(0)1815057937, FAX ++44(0)1715945255
                            lag                   ellum
                             f   e               lag 
       ecoliecoliecoliecoliecoli  l            mf 
      cecoliecoliecoliecoliecolii  l          u 
      eecoliecoliecoliecoliecolic   umf     ll      
      coecoliecoliecoliecoliecoli      l  ge 
       ecoliecoliecoliecoliecoli        a         
         p                   f       fl g 
        i                   l     lum   el
       l                     agel         lu
      u                                      mflagellum

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