Competent BL21 Cells?

Peter Erhard perhard at geronimo.drad.umn.edu
Wed Jan 25 21:55:02 EST 1995


In article <3fn1nk$d9g at server.st.usm.edu> jstemple at whale.st.usm.edu (Jeffrey Shane Temple) writes:
>
>I was wondering if there was anyone out there with enough experience in
>making competent that could help me!!  I am trying to make some BL21 cells
>competetent.  The protocol that I have is a modified Hanahan (?)
>procedure--using cobalt.  What I want to know is if someone could offer me
>a simpler protocol for making competent cells.  Any help would be greatly
>appreciated.
>
>Jeffrey Temple
>Univ. of Southern MS
>Department of Chem/Biochem

Hi Jeffrey,
you could look into Chung CT, Henrick K. PNAS 86 2172 1989
regards
Catherine on Peter Erhards account




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