Reference for the toothpick PCR technique?
shopkins at fs1.ho.man.ac.uk
Wed Jan 25 17:48:05 EST 1995
> using a toothpick for re-amplification of a PCR
your recently posted method for isolating biotinylated PCR products for re-PCR.
How necessary is this? To get a lot of ß-actin cDNA for working up a
quantitation method we just centrifuged the PCR product in an Amicon Centricon
10 or 30KDa cut-off device and used that. Seems to be O.K. Were we being
horribly naive, or lucky?
More information about the Methods