2D-PAGE membrane proteins

[Christer Ericsson]

I am attempting to analyze a group of integral membrane proteins, using
2D-PAGE. While I know that standard 2D-PAGE sample buffer works (to some
degree of ³works²), I am wondering if anybody made a systematic
investigation into what would be the *best* sample buffer for integral
membrane proteins generally?

Sincerely

Christer

-- 
Christer Ericsson, Dept. of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, S-171 77 Stockholm, Sweden. E-mail: christer.ericsson at mog.ki.se.