Viraj A. Master
vam2 at midway.uchicago.edu
Thu Jan 26 20:18:11 EST 1995
In article <3g3l50$al0 at lyra.csx.cam.ac.uk>, cdw1000 at mole.bio.cam.ac.uk
(Christopher Walentas (Bioc)) wrote:
| Flipping through the FMC booklet that comes with NuSeive,
| I saw they had a protocol for in-gel ligations and
| transformations. Is it possible to use electroporation
| with a gel slice as well or due to the electrophoretic
| buffers (salt) is one stuck the Hanahan and the like?
| Any advice on the subject that you could forward to me
| (incl. other sources to chase down) would be appreciated.
| Cheers in advance,
I do in-gel ligations according to the protocol of
Kalvakolanu and Livingston (1991) Biotechniques 10:176
and then use 4microliters of the diluted ligation (1:1 with water) for
electroporation (using 80microliters of cells). It works well.
Department of Organismal Biology and Anatomy
University of Chicago
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