multi-primer PCR successful

Michael Cooley szcooley at dale.ucdavis.edu
Thu Jan 26 18:31:11 EST 1995


Darren M. Stauth (stauth at crunch.usgmrl.ksu.edu) wrote:
: I heard some discussion earlier about using multiple primers (more than
: two) in PCR screening.  So I tried it out and it worked beautifully.  I
: tested two cocktails with a total of 7 primers (one common to all of my
: samples and two sets of six unique primers).  I used the program Amplify to
: make sure all primers were compatible.  Each primer was at a final
: concentration of 10pmol for a 10ul reaction (used a hot-air termo-cycler). 
: I made sure to have positive and negative controls for each of the 12
: unique primers.  It was a total success!  A couple artifacts from
: misannealing do to the high concentration of primers were observed, but
: they were easily distinguished from true positives.  This means a major
: saving of time and effort for me.  Just thought I'd pass on the news to
: anyone interested.  Feel free to write for any info.  


Where does this program Amplify come from?


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