Source of restriction enzyme CviJI
curtis at UTMSI.ZO.UTEXAS.EDU
Thu Jan 26 12:10:31 EST 1995
The enzyme is produced by MegaBAse Incorporated of Lincoln, Nebraska.
Sorry, don't have a phone number, but I've forwarded your message to a
colleague in Lincoln who will be able to provide the information.
Q | \
| \ |_____OO\________ Curtis Suttle
|__\___________/________/______/ Marine Science Inst
\ o o o o LONGHORN O / Univ of Texas @ Austin
\ VV/ Port Aransas TX 78373
~~~~~~\~~~~~~~~~~~~~~~~~~~~~~~~/~~~~~ curtis at utmsi.zo.utexas.edu
On 26 Jan 1995, Bill Kimmerly wrote:
> At the DOE Human Genome Contractor's Meeting in Santa fe this
> past November I heard a talk discussing the use of an enzyme called
> CviJI in creating "random" blunt-end fragments from a large target.
> The enzyme's specificity was RC/GY > RC/GR > YC/GY. Yes, I know
> it is not truly random, nevertheless I am interested in exploring its
> utilty in library construction. Does anyone know of a commercial source
> of this enzyme? Any first-hand experience, recommendations, do's & don't's
> are, of course also welcome. Thanks in advance for any help.
> Bill Kimmerly
> Staff Scientist
> Human Genome Center
> Lawrence Berkeley Laboratory
> kimmerly at genome.lbl.gov
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