Source of restriction enzyme CviJI

Thu Jan 26 12:10:31 EST 1995

The enzyme is produced by MegaBAse Incorporated of Lincoln, Nebraska. 
Sorry, don't have a phone number, but I've forwarded your message to a 
colleague in Lincoln who will be able to provide the information.

   Q              |  \
   |\             |---\--\  
   | \            |_____OO\________        Curtis Suttle
   |__\___________/________/______/        Marine Science Inst
    \ o o o o         LONGHORN O /         Univ of Texas @ Austin
     \                        VV/          Port Aransas  TX 78373
~~~~~~\~~~~~~~~~~~~~~~~~~~~~~~~/~~~~~  curtis at

On 26 Jan 1995, Bill Kimmerly wrote:

> At the DOE Human Genome Contractor's Meeting in Santa fe this
> past November I heard a talk discussing the use of an enzyme called
> CviJI in creating "random" blunt-end fragments from a large target.
> The enzyme's specificity was RC/GY > RC/GR > YC/GY.  Yes, I know
> it is not truly random, nevertheless I am interested in exploring its
> utilty in library construction.  Does anyone know of a commercial source
> of this enzyme?  Any first-hand experience, recommendations, do's & don't's
> are, of course also welcome.  Thanks in advance for any help.
> Regards,
> Bill Kimmerly
> Staff Scientist
> Human Genome Center
> Lawrence Berkeley Laboratory
> kimmerly at

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