22346BSS at MSU.EDU
Fri Jan 27 17:09:35 EST 1995
I have been trying to ligate:
1- A 7.5 kb SmaI fragment into a 7.5 SmaI digested vector. Digestion
was done at room temperature.
2- A 7.5 kb ASP718/BamHI fragment into a 10 kb baculovirus vector
and a 13 kb plant binary vector.
Vectors were dephosphorylated. Before ligation, inserts and and vectors
were purified after running in an agarose gel using GeneClean kit.
I have tried several conditions but with no success.
If anyone have experience in cloning large fragments into large vectors, I
would appreciate any suggestions.
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