Partial Digests:I need advice!

Heidi Moss hmmoss at MAIL.MED.CORNELL.EDU
Fri Jan 27 12:19:55 EST 1995


I am trying to map several human genomic fragments in lambda and would like 
any advice regarding partial digests: is there any trick? What should I 
look for in terms of a "good" partial vs a "bad" partial? I am using 
Stratagene's non-rad gene mapping system which uses T7/T3 oligo probes 
and partial digests to find the restriction sites. 
Any advice would be appreciated!
Thanks!
-- Heidi



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