help about adaptor synthesis
brad at corona.med.utah.edu
Sat Jan 28 18:29:49 EST 1995
In article <3gbo9d$3uu at news.iastate.edu>, qfdong at iastate.edu (Qunfeng Dong)
> Does anyone have any experience to synthesize adaptor?
> Thanks in advance!
See "Use of conversion adaptors to clone antigen genes in lambda gt11."
Stover, Vodkin & Oaks. 1987. Analytical Biochemistry 163, 398-407.
Basicly they mixed complementary oligos in equimolar concentrations (the
duplex DNA concentraion adjusted to 20 microM) in 25 miliM Tris-HCL 8.0, 10
miliM MgCl2. They then heated the mixtures to 95 C and cooled to room
temperature slowly (~ 1 hr.) [Taken near verbatim from p. 400 of article.]
Mistakes are mine and not the authors.
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
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or: (801)-581-4365 | My opinions are solely my own.
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