PCR single copy genes

Rob Jordan jordan at mbcrr.harvard.edu
Fri Jan 27 07:37:35 EST 1995


Does anyone have any advice for PCR amplification of single copy genes
from genomic DNA for analytical purposes. 

How much genomic DNA should I use in the reaction?

What concentration of primers?

Any helpful hints. Thanks!

Rob J.

-- 
Anh Nguyen
anh_nguyen at macmailgw.dfci.harvard.edu



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