PCR single copy genes

Martin Leach leach at bu.edu
Mon Jan 30 10:38:52 EST 1995

In article <jordan-2701950737350001 at mac-burl-11.dfci.harvard.edu>,
jordan at mbcrr.harvard.edu (Rob Jordan) wrote:

> Does anyone have any advice for PCR amplification of single copy genes
> from genomic DNA for analytical purposes. 
> How much genomic DNA should I use in the reaction?
> What concentration of primers?
> Any helpful hints. Thanks!

when really stuck...10% DMSO...or 10%DMF sometimes help.....alternatively
NP-40 or TWEEN 20 (forgot concs of these..)



.....          Martin Leach                Email:leach at bu.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
   / o  /      Boston Univ. School of Med. Fax:   (617) 638-4329         
 _/  |-/__==/  80 E. Concord St. (L603)
(BULLDOZER) \_ Boston MA 02118            "Not the old underpants on your
               USA                           head.....WIBBLE" -BLACKADDER 
p.s. try BioMOO (virtual biology on the internet - telnet bioinfo.weizmann.ac.il 8888)
My home page:


More information about the Methods mailing list