PCR single copy genes

Martin Leach leach at bu.edu
Mon Jan 30 10:38:52 EST 1995


In article <jordan-2701950737350001 at mac-burl-11.dfci.harvard.edu>,
jordan at mbcrr.harvard.edu (Rob Jordan) wrote:

> Does anyone have any advice for PCR amplification of single copy genes
> from genomic DNA for analytical purposes. 
> 
> How much genomic DNA should I use in the reaction?
> 
> What concentration of primers?
> 
> Any helpful hints. Thanks!
> 

when really stuck...10% DMSO...or 10%DMF sometimes help.....alternatively
NP-40 or TWEEN 20 (forgot concs of these..)

Martin

-- 

.....          Martin Leach                Email:leach at bu.edu 
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