Is pcr derived sequence acceptable for publication?

Tracy Aquilla aquilla at salus.med.uvm.edu
Mon Jan 30 08:46:30 EST 1995


In Article <1C6F50A0B5F at rose.le.ac.uk>, meh2 at leicester.ac.uk ("M.E. Hudson")
wrote:
>I am planning to isolate cDNA sequence which may at some point be 
>used in transforming plants. It would be necessary to publish the 
>sequence if the work was ever published. Consequently, I have been 
>warned off using pcr methods such as inverse pcr or amplifying cDNA 
>by using homopolymer tailing and oligo dT + dC primers. Is it true 
>that pcr sequence is regarded as dubious, does this apply to pcr 
>using polymerases with proofreading activity and to conventionally 
>cloned fragments sequenced by pcr, and is the taq error rate 
>significant compared to that of dideoxy sequencing?
>
>Thanks in advance. 

If yo search the databases, I think you will find many genes which were
cloned by PCR.
    Tracy



More information about the Methods mailing list