Is pcr derived sequence acceptable for publication?
aquilla at salus.med.uvm.edu
Mon Jan 30 08:46:30 EST 1995
In Article <1C6F50A0B5F at rose.le.ac.uk>, meh2 at leicester.ac.uk ("M.E. Hudson")
>I am planning to isolate cDNA sequence which may at some point be
>used in transforming plants. It would be necessary to publish the
>sequence if the work was ever published. Consequently, I have been
>warned off using pcr methods such as inverse pcr or amplifying cDNA
>by using homopolymer tailing and oligo dT + dC primers. Is it true
>that pcr sequence is regarded as dubious, does this apply to pcr
>using polymerases with proofreading activity and to conventionally
>cloned fragments sequenced by pcr, and is the taq error rate
>significant compared to that of dideoxy sequencing?
>Thanks in advance.
If yo search the databases, I think you will find many genes which were
cloned by PCR.
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