A GOOD KIT FOR PCR CLONING
drm21 at mole.bio.cam.ac.uk
Mon Jan 30 08:07:00 EST 1995
In article <reedc-2901952101470001 at ip043167.iac.net>, reedc at iac.net (Reed
R. Corderman) wrote:
> In article <Pine.ULT.3.91a.950126165357.24016A-100000 at kepler.unh.edu>,
> xz at KEPLER.UNH.EDU (Xin-Hua Zhou) wrote:
> > I am looking for a good kit for PCR cloning. Can you give me some ideas
> > , based on your EXPERENCE?
> The best so far for me is the TA cloning kit from Invitrogen. With a 25
> microliter PCR I use 2 to 4 microliters directly in 25 microliters of
> competent cells. Follow their transformation protocol, plate the entire
> transformation on two plates and voila!- Bunches of white colonies and a
> few blue ones.
Er. Maybe I'm missing something here. Don't you need a ligation in there
somewhere between the PCR and the transformation? If not, I'd be very
interested to hear the protocol!
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