luciferase clone wanted

Randy Haun rhaun at nih.gov
Tue Jan 31 11:27:19 EST 1995


In article <9500307915.AA791514934 at tcplink.nrel.gov> roesslep at TCPLINK.NREL.GOV writes:
>From: roesslep at TCPLINK.NREL.GOV
>Subject: luciferase clone wanted
>Date: 30 Jan 1995 16:15:13 -0800
>     
>     Has anyone by chance constructed a luciferase gene that has an NcoI 
>     (or compatible) site on the 5' end?
>     
>     Cheers,
>     
>     Paul Roessler
>     National Renewable Energy Lab
>     Golden, Colorado USA
>     
>     paulr at nrel.gov
>

Do you want to use this construct for promoter analysis (i.e., are you going
to clone a putative promoter element 5' to the luciferase gene and measure
luciferase activity after transfection)?  If so, I have a luciferase 
construct that can be used for this purpose that does not rely upon the
presence of any particular restriction site.  The method requires the 
amplification of the promoter element using vector specific primers.  If you
are interested in the method/vector, let me know.

Randy Haun
NHLBI/NIH
rhaun at nih.gov



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