DNA elution from gels
labonnes at csc.albany.edu
Tue Jan 31 14:12:37 EST 1995
In article <D38tJK.IDz at ncifcrf.gov>,
Paul N Hengen <pnh at fcs260c2.ncifcrf.gov> wrote:
> In article <3gbs9n$n6t at rebecca.albany.edu> labonnes at csc.albany.edu
> (S. LaBonne) writes:
>| I assume you mean agarose gels. Having tried lots of methods for
>| extracting bands from agarose gels, my current favorite is digestion
>| with GELase (Epicentre Technologies, Madison, WI; 1-800-284-8474).
>| In my hands it reliably gives very good yields of clean DNA, good for
>| sequencing, cloning or whatever.
>Have you been able to PCR amplify DNA extracted in this way? Past discussions
>here concluded Gelase extracted DNA does not work for PCR. I assume you are not
>cycle sequencing :-o
I haven't cycle-sequenced, but I _have_ amplified successfully. I
_think_ other people in my lab have successfully performed dye-deoxy
sequencing on GELase-purified DNA; I'll have to check that. One
problem variable (for _any_ extraction method) might be the quality
of the LGT agarose used; I've always used Fisher's.
Steve LaBonne *********************** (labonnes at csc.albany.edu)
"It can never be satisfied, the mind, never." - Wallace Stevens
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