mRNA PolyA tail ??
smori at nmsu.edu
Sun Jan 29 17:12:59 EST 1995
Dr. YL Du (yd128 at albnyvms.bitnet) wrote:
: Questions about the poly A tail of RNA in mammalian cells.
: 1. Is it true that every mRNA must have a poly A tail? Has anybody
: ever find a cDNA without a poly A tail instead you find a AT rich region
: at the 5 prime end? If you find such cDNA form cDNA library screen,
: would you think it a cDNA from mRNA? Any comment.
No. some histone mRNA's are not polyadenylated. But they have some unique
sqces at their 3'end that form a stem and loop region followed by a
purine-rich region. And if you are looking to isolate cDNA using PolydT's you
would miss such messages.
Shahram Mori _/\_
Program in Molecular Biology _\ /_
Dept. of chemistry and Biochemistry Box 3C \_ _/
NMSU Las Cruces NM ||
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