Sat Jul 1 16:01:19 EST 1995
In article <theveno-300695142147 at cc-hsc-k-box20.cc.umanitoba.ca>,
theveno at ccu.umanitoba.ca (Tracy) wrote:
> I am attempting to sequence a 264 bp region. I am loading the gel two
> times: the first loading runs for 2.5 hours and then I do the second
> loading and let the gel run for another 2 hours ( ie the first loading runs
> a total of 5.5 hours). The problem is that on film the first loading is
> all smeared but the second loading is ok. I know the gel is ok and my
> samples are ok because the second loading is fine. I am running the gel
> at constant power 55 W. Any ideas?
> Thanks in advance
are you pre-running your gel before you do your first loading? I always
used to pre-run my gels for half an hour (at 40-45 W constant power)
before loading my samples. Hope this is of some help.
(ksnowden at bio.tamu.edu)
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