laying membranes on bacteria plates

R.D.Johnson R.D.Johnson at massey.ac.nz
Tue Jul 4 00:21:00 EST 1995


A quick (not dirty) method is as follows.

Place nylon membrane on the agar plate surface. 
plate the bacteria directly on the membrane at dilution of choice.
Grow overnight or as usual until good colonies are clear.
Lift filters from plate and place (bacteria side up) on a few Mls
denaturation soln on a bench for 2 minute.
Transfer filter to a few Mls of neutralisation soln and leave 5 min.
remove filter allow to dry and then back in the microwave at high
power for one minute to fix DNA.
Filter is ready to probe.

Good luck

Rich





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