Stripping Hybond membranes

Jeffrey Smerage smerage at cmg.health.ufl.edu
Mon Jul 3 12:43:51 EST 1995


I regularly strip Hybond membranes, and yes you should loose a little of
your DNA.  I have never tried to quantitate my losses although they don't
make a significant different difference in my subsequent exposure times.

I uses a 0.1X SSC, 0.1% SDS solution at 95°C twice to strip my membranes. 
This came from a Biotechniques article titled "Evaluation of DNA Probe
Removal from Nylon Membrane", Biotechniques, 1992, 13:572-575.



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