Boomerang DNA Amplification Images

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Mon Jul 3 14:16:51 EST 1995


Some people were trying to contact me about getting the Boomerang DNA
amplification images which originally came from Kevin Ahern
(ahernk at bcc.orst.edu). However, since he sometimes does not reply to personal
e-mail, you can now get the information from his form letter (boomerang.txt)
explaining the history of the boomerang technique and the self-explanatory
picture (either bda.gif or bda.tif) from my ftp site at ftp.ncifcrf.gov in the
directory pub/methods/boomerang. Interestingly, the method only requires one
primer and is therefore NOT covered by the existing U.S. patent on DNA
amplification using a set of two primers (PCR). For additional information on
similar techniques, see the references below. Happy Boomeranging!!!

@article{Arnold1991,
author = "C. Arnold
     and I. J. Hodgson",
title = "Vectorette {PCR}: a novel approach to
genomic walking",
journal = "PCR Methods and Applications",
volume = "1",
pages = "39-42",
comment = "vectorette PCR",
year = "1991"}

@article{Devon1995,
author = "R. S. Devon
     and D. J. Porteous
     and A. J. Brookes",
title = "Splinkerettes -- improved vectorettes
for greater efficiency in {PCR} walking",
journal = "Nucleic Acids Res.",
volume = "23",
number = "9",
pages = "1644-1645",
comment = "vectorette PCR",
year = "1995"}

*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - - - - Methods FAQ list -> http://www-lmmb.ncifcrf.gov/~pnh/ - - - - - - *
* - - -  Anonymous FTP from ftp.ncifcrf.gov as file pub/methods/FAQlist - - - *
*******************************************************************************



More information about the Methods mailing list