DNA purification from agarose gels
xunfang at wvnvms.wvnet.edu
xunfang at wvnvms.wvnet.edu
Mon Jul 3 21:35:58 EST 1995
In article <3sp9rk$1tp at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk (R. Woodward) writes:
> Dear all
> I would like to canvas opinion on the best kit or perhaps method to use
> when purifying DNA fragments from agarose gels, at the moment we use the
> 'EluQuik' kit from S&S the yield is good and so is the purity but its
> alittle fiddly especially if you perform alot of isolations at one time
> . I want to use the purified fragments in ligation reactions.
> Thanks to all Robert
>
> R.Woodward
> Dept Pharmacology
> Tennis Court Rd
> University of Cambridge
> Email rw200 at cus.cam.ac.uk
Try Qiaquick Gel Extraction Kit from Qiagen. It takes only 20 minutes for
one sample. The recovery is around 50% or more.
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