Isol. of Transcription factors

Bernhard Schrautemeier schraute at uni-bonn.de
Mon Jul 3 11:48:38 EST 1995


I am looking for a method for positive selection of (cyano)bacterial
transcription factors expressed in (E. coli) gene libraries to isolate the
corresponding gene.  

Also I heard that it is possible to bind promoter-containing DNA sequences
to certain matrices (e. g. latex; J. Biomater. Sci. Polym. Ed. 5(4),
293-302, 1994; I don´t have access to this journal) and thus to
selectively purify binding proteins, e.g. for use in footprinting.

I would be glad to get some help!

-- 
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Bernhard Schrautemeier      Tel: +49-228-73-5277 or 5536
Botanisches Institut            Fax: +49-228-73-5513
der Universitaet Bonn        E-mail: schraute at uni-bonn.de
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53115 Bonn                 x
Germany                    x
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